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Similarly, the combinations of substrates that gave the highest PDAT1 activity were also those that produce the two TAG species (547 and 548 TAG) with the highest increase in PDAT overexpressing C. sativa seeds. Thus, the in vitro data correlate well with the changes in the overall fatty acid profile and TAG species in C. sativa seeds with altered DGAT1 and PDAT activity. Additionally, in vitro studies of C. sativa phosphatidycholinediacylglycerol cholinephosphotransferase (PDCT), another activity involved in TAG biosynthesis, revealed
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