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Added Cu2+ to H2BS can cause fluorescence quenching. Further experiments showed that H2BS and Cu2+ form a stable 12 complex, while B4O72- can also cause fluorescence quenching of H2BS, which is the occurrence of the PET effect. Meanwhile, H2BS can be used for quantitative detection in the environment and rapid identification in life. Transplantation of HIV-positive (HIV+) donor organs for HIV+ recipients (HIV D+/R+) is now being performed as research in the United States, but raises ethical concerns. While patient-reported outcome meas