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A significant infestation rate of 5525% was found in 163 out of 295 infested hosts, leading to the collection of a total of 409 H. kumari ticks. The collection yielded 204 females, 158 males, and 47 nymphs. The infestation of goats stood out significantly, with 224 ticks causing an infestation rate of 5833% (98/168). A markedly lower infestation was observed in sheep, which had 185 ticks with a rate of 5118% (65/127). Tick genomic DNA was employed to amplify partial genes of t