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Hierarchical clustering analysis, in addition, showed a substantial upregulation of genes associated with lipid metabolism, oxidative stress response, and innate immunity in CyB-exposed zebrafish. Ultimately, CyB provoked liver damage and heightened apoptosis of hepatocytes in zebrafish, thus raising the protein expression levels of Bax, TLR4, NF-κB p65, and STAT3. The final result of inhibiting the TLR signaling pathway, achieved by knocking down TLR4, is a considerab