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Our results open up new perspectives for behavioural studies on manatees, in particular those associated with cognition, management and welfare in captivity.Xanthium italicum is an aggressive weed found worldwide. Despite several ecological, morphological, and physiological research on its invasion mechanism, the mechanism of its successful invasion has not been revealed from the viewpoint of population genetics. Thus, we aimed to evaluate the genetic variation within and among populations of the alien invasive weed X. italicum in China