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The one step tritiation of S-(4-nitrobenzyl)-6-thioinosine is described with characterization of the product by tritium NMR as well as mass spectrometry. The storage, stability and repurification of [benzyl methylene-3 H]S-(4-nitrobenzyl)-6-thioinosine are also discussed. This article is protected by copyright. All rights reserved.Testing for bioluminescent pyrophosphate is a convenient method of DNA detection without complex equipments, but it is insufficiently sensitive and offers no particular time advantage over other rapid de