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Short-term heat stress during male meiosis causes defects in crossover formation, meiotic progression and cell wall formation in the monocot barley, ultimately leading to pollen abortion. High temperature conditions cause a reduction of fertility due to alterations in meiotic processes and gametogenesis. The male gametophyte development has been shown to be particularly sensitive to heat stress, and even short-term and modest temperature shifts cause alterations in crossover formation. In line with previous reports, we observed that ma