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© 2021 Wiley Periodicals LLC. Basic Protocol Measurement of cellular ECAR, OCR, and ATP production in live HepG2 cells Support Protocol 1 Culturing and maintaining of HepG2 cells Support Protocol 2 Determining optimal cell density per well. Pouchitis is a condition with large unmet medical needs and no approved therapies. Lack of validated instruments to measure disease activity and treatment response is a major barrier to drug development. To conduct a modified RAND/University of California Los Angeles appropriateness process to produce
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