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The Tax4Fun package was employed to determine the potential functional pathways. Among the 3277 operational taxonomic units (OTUs) observed, the Kongbai group exhibited 2410, the Gengzu group 2236, and the Yinliu group 1763. Rats with obstructed bile ducts demonstrated a variance in the microorganism composition, encompassing classification levels such as phylum, class, order, family, and genus. Following biliary drainage, the comp