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Coronary perforations were more common with stiff whereas wire fracture was more common with soft polymer-jacketed guidewires. The most common failure modes of polymer-jacketed guidewires during percutaneous coronary intervention are failure to cross the lesion, guidewire fracture, and peeling of the polymer jacket. Coronary perforations were more common with stiff whereas wire fracture was more common with soft polymer-jacketed guidewires.Monitoring cell growth and target production in working fermentors is important for stabilizing high