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87-6.1 μmol/L and the reference interval 1.28-19.67 μmol/L (2.5th-97.5th percentile). In women median TMAO plasma concentration was 3.56 (Q1-Q3 2.41-5.15) μmol/L and the reference interval 1.08-17.12 μmol/L. In multivariable regression analysis plasma TMAO was associated with ****, renal function and diet. The association of TMAO and diet was significant for intake of fish and shellfish in men only. Conclusions In a community-based sample free of apparent CVD and renal disease, we report the distribution of TMAO plasma concentrations wit