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Investigating Plasmodium berghei malaria parasites, in an asynchronous state, within mice offers a route to discovering new antimalarial medications. To assess the effectiveness and mechanism of action of drugs, it is important to conduct studies on a specific stage of progression. Endoperoxide 6-(12,67-tetraoxaspiro[711]nonadec-4-yl) hexan-1-ol (N-251) served as a model antimalarial compound in our study involving P. ch