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Secondary metabolites, a crucial defense strategy for benthic invertebrates against consumers, enhance their fitness levels. An investigation of the chemical constituents of Ophiopholis mirabilis was performed to reveal the chemical defense mechanisms in marine benthic echinoderms. The initial isolation of asterosaponin P1, a steroidal monoglycoside, from the organism O. mirabilis was executed via the method of column chromatography. Spectroscopic and spectrometric met
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