https://nkl22.com/most-india-h....ard-air-passage-affi
Saturation mutagenesis of the N-terminal coding sequences of hemoglobin genes, combined with high-throughput fluorescence-based screening, led to the generation of variants displaying a 166- to 345-fold increase in hemoglobin expression. To preclude the use of toxic inducers, like isopropyl and D-thiogalactopyranoside, two naturally inducible expression systems were formulated, utilizing the food additives propionate and gluconate
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