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Background Invasive plant species present a serious threat to environment and, human and animal health. An interaction may exist between the climatic changes and invasive plant species. In this two-year study, we investigated effects of warming, CO2 and nitrogen application on biomass, growth and leaf tissue nitrogen concentration of three invasive weed species. Treatments were i) simulated (elevated) CO2 (~800-900 ppm); ii) warming or high temperature (day/night 25/15°C); iii) simulated (elevated) CO2 combined with high temperature (