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Intestinal electrical activity, the rectus abdominis electromyogram (EMG), and the electrocardiogram (ECG) were all recorded by the PowerLab data acquisition and analysis system. The serum concentrations of cAMP and cGMP were determined using ELISA. The expression levels of colonic tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) proteins were determined using immunofluorescence staining and Western blot analysis, respectively
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