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An indirect solid-phase ELISA employing VP2/VP6 rotavirus VLPs as the antigen was constructed to measure and evaluate the spread of RVA-specific IgG, IgM, and IgA in the rotavirus A immune response. Rotavirus VP2/VP6 VLP production and purification were studied using electron microscopy, PAGE, immunoblotting, ELISA, and a virus neutralization assay. The study reports on the construc
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