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Community members, selected as part of a wider investigation into knee pain, contributed demographic, clinical, and pain-related data, and an intravenous blood sample to measure DNA methylation levels at CpG locations. A comparison of DNA methylation patterns revealed differences in genes crucial to vitamin D metabolism (including CYP24A1, 24-hydroxylase and CYP27B1, 1-hydroxylase) between those with and without pain. Hypermethylation was additionally present on the gene that specifies the Vitamin D receptor (VDR). Th