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Zearalenone (ZEA) exposure is linked to carcinogenic outcomes in human and animal health, with notable repercussions for the intestinal, hepatic, and renal systems. A complete understanding of how ZEA initiates apoptosis and tissue damage remains elusive at present. This research project aimed to determine genes involved in modulating cellular responses to ZEA, using CRISPR-Cas9 screening, and subsequently validating the functions of novel genes to further illuminate the underlying molecular mechanisms of specific b