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Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), has been recently demonstrated to be heteroecious and macrocyclic. The pathogen depends on wheat as the primary (telial) host and barberry as the main alternate (aecial) host to complete the complete life cycle. Viable teliospores are essential for the initiation of ****ual reproduction of Pst. However, there is no exact source of viable teliospores are discovered yet to produce basidiospores to initiate infection on susceptible barberry. In the present study, we inves