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5%. Our results demonstrated that PLGA/PEI NPs encapsulation of GFP mRNA had no toxic effect on immature monocyte-derived dendritic cells and was capable of delivering of IVT-mRNA into moDCs and was highly effective. The expression of GFP protein 48 h after transfection was confirmed by flow cytometry, microscopic examination and western blotting assay. This NP can make a way to target moDCs to express a variety of antigens by IVT- mRNA. The present study introduced the PLGA/PEI NP, which provided effective delivery of IVT-mRNA that
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