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In conclusion, it is here reported for the first time that ZnO NPs induce cytotoxic effects, including changes in cytoskeleton and nucleoskeleton in mouse spermatogonia cells, which may compromise the progression of spermatogenesis in a time- and dose-dependent manner.The removal of antibiotic growth promoters (AGPs) as feed additives in poultry nutrition from the market in many countries has compelled researchers to find unconventional and safe alternatives to AGPs. Probiotics, prebiotics, enzymes, organic acids, herbs, immune-stimulants