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The complete analytical pipeline was then used to immunophenotype the local inflammatory infiltrate in individuals with and without acute bacterial infection. CytoPy is open-source and licensed under the MIT license. CytoPy is available at https//github.com/burtonrj/CytoPy, with notebooks accompanying this manuscript (https//github.com/burtonrj/CytoPyManuscript) and software documentation at https//cytopy.readthedocs.io/.BACKGROUND Vancomycin is an antibiotic commonly used for management of severe gram-positive infections. It is infrequ