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By the conclusion of a seven-day biofilm formation period, the metabolic activity (MA) was measured with 23,5-triphenyltetrazolium chloride, and the biofilm's configuration was identified through Checker-board DNA-DNA hybridization. Statistical analysis involved the Kruskal-Wallis test, subsequently scrutinized by Dunn's post-hoc test. Protocol A demonstrated that all treatments reduced MA levels compared to the placebo, a finding supported by a
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