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Phase-resolved luminescence chemical sensors provide the analyte determination based on the estimation of the luminescence lifetime. The lifetime is estimated from an analysis of the amplitudes and/or phases of the excitation and emission signals at one or several modulation frequencies. This requires recording both the excitation signal (used to modulate the light source) and the emission signal (obtained from an optical transducer illuminated by the luminescent sensing phase). The excitation signal is conventionally used as reference,