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Research investigates the intricate interplay of two star polymers, fabricated using poly(2-(dimethylamino)ethyl methacrylate) as a building block, with varying nucleic acid configurations. Star polymers are differentiated solely by their ability to accommodate protonatable amino groups or permanently charged quaternary ammonium groups, while DNAs exhibit diverse molar masses, lengths, and topological configurations. The polyplexes' crucial physicochemical parameters are identified. The impac