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Previous research documented the substantial expression of enhancer of zeste homolog 2 (EZH2) within ATC, potentially identifying it as a therapeutic target, but the influence of EZH2 on ATC growth remains empirically undetermined. An investigation into the impact of an EZH2 inhibitor, DZNep, was conducted on four ATC cell lines: 8305C, KTA1, TTA1, and TTA2. All ATC cell lines underwent a g