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A meticulous double-checking of the target miRNA sequence first within the CRT and subsequently within the CRISPR system is the rationale behind the assay's remarkable specificity in identifying miR-21. Through the employment of this assay, a sensitive measurement of miR-21 levels in human serum samples has been successfully carried out, enabling the identification of diseases present in the human serum samples. Inarguably, the field of clinical molecular diagnosi
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