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A pervasive global health challenge, emerging infectious diseases profoundly affect both human and animal health. Mosquito-borne transmission of West Nile virus (WNV) between birds, a vector-borne virus, can also infect humans and horses, resulting in health complications. Spain has a long history of local West Nile Virus circulation, and since 2010, equine populations have experienced frequent outbreaks. Astonishingly, only six cases were reported in humans until the year 2019. A substa