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plication and dissemination in Ornithodoros ticks are major mechanisms underlying ASFV horizontal and vertical transmission. However, our results indicate that other determinants beyond viral ****tion also influence ASFV vector competence. Further research is required to fully understand this process in soft ticks. This study provides molecular data indicating that viral ****tion and dissemination in Ornithodoros ticks are major mechanisms underlying ASFV horizontal and vertical transmission. However, our results indicate that othe