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Ribosomal internal transcribed spacer molecular analysis pinpointed it. To determine the effects of various nitrogen and phosphorus concentrations on biomass and lipid production, a single-factor design was implemented using 250-mL Erlenmeyer flasks. Response surface methodology was applied to a 32-factorial design manipulating light intensity and CO2, within 200 mL tubular photobioreactors (PBRs), to investigate its impact on biomass, lipid product