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After 7 days of the administration, the preparation can promote the proliferation of conjunctival goblet cells and down-regulate the cornea expression levels of MMP-3 and MMP-9 in epithelial cells. The LCTG has a good curative effect on mice with DED, and the overall curative effect is better than that of levocarnitine solution. The LCTG has a good curative effect on mice with DED, and the overall curative effect is better than that of levocarnitine solution.In this study we aim to identify the optimal cellulose extraction protocol for 14