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The ALPPS group treated with L-NAME and the PVL group exhibited no divergence in FLR/BW ratio and Ki-67 labeling index. In the molsidomine-treated PVL group, an increase in the FLR/BW ratio and Ki-67 labeling index was observed, mirroring the level seen in the ALPPS group. Although the early release of inflammatory cytokines may not be essential for the rapid recovery of the FLR after ALPPS, activation of the Akt-eNOS pathway could play a vital role in the accelerated regeneration process. Accelerated FLR regeneration after ALPPS may not depend on the ear