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The opening of the blood-brain barrier (BB was accomplished using a 1 MHz preclinical ultrasound system and 10 L/g microbubbles. An evaluation of immune cell populations and the extent of immune checkpoint inhibitor brain penetration was carried out using flow cytometry. The study examined how frequently applied treatments influenced survival durations. Immunocompetent mice, bearing GL261 syngeneic tumors, showed that UMBO consistently and safely reopened the blood-brain barrier. Employing Evans blue dye and magnetic resonance imaging, the opening of the B
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