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A homemade humic acid column, operating at an exceptionally low backpressure (0.60 MPa at a 20 nl/min flow rate), exhibited a lengthy lifespan, outstanding reproducibility, and a negligible incidence of nonspecific binding. A series of known humic acid ligands, including rodenticide molecules, were used in a recognition assay to demonstrate the efficacy of this affinity humic acid column, a key element in fragment-based drug design. This col