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Isolated from wastewater in Valparaiso, Chile, the polyvalent bacteriophage fp01 demonstrated lytic activity across a range of bacterial species, such as Escherichia coli and Salmonella enterica serovars. The polyvalent nature of bacteriophage fp01 suggests its suitability for biomedical, food, and agricultural applications. Polyvalent bacteriophage biology's fundamental intricacies are yet to be fu