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Several genes from immune cells, CXCL13, CXCR5, and PADI4, were found to serve as novel biomarkers associated with a poor clinical prognosis. Among the findings of this study was a novel subcluster of malignant cells, marked by early metastasis and angiogenesis, which presented a significant departure from the progressive trajectory of ESCC. Analysis of intercellular interactions, using ligand-receptor pairs, identified a subcluster of malignant cells interac