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The nucleotide sequencing analysis of the positive controls used in this study showed a close homology with the reference gene bank sequences of 3D7 (CPO16995.1) and Sal-1(UO3079.1). This study revealed a low frequency of asymptomatic malaria trend within malaria endemic areas of southeastern Iran which are under intense elimination program and also the ability of HRM assay in detecting low spp parasitemia beyond the limits of microscopy and RDTs. This study revealed a low frequency of asymptomatic malaria trend within malaria endemic ar
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