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Among seven phytopathogenic fungi, (-)-(1S,4R)-benzovindiflupyr displayed greater potency than (+)-(1R,4S)-benzovindiflupyr, as assessed via a stereoselective bioactivity assay. Molecular docking studies indicated that (-)-(1S,4R)-benzovindiflupyr demonstrated a more potent binding interaction with succinate dehydrogenase than its enantiomer, (+)-(1R,4S)-benzovindiflupyr. Mutated succinate dehydrogenase with the H272( substitution in phytopath