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This paper reports single-molecule tracking (SMT) measurements of the diffusion behaviors of individual, anionic sulforhodamine B (SR dye molecules in a series of poly(ethylene oxide) (PEO) films, aimed at clarifying the influences of the molecular weight, network plasticization, and thermal annealing on such dynamics. Micrometer-thick PEO films were prepared by drop-casting from its aqueous (0.2%, 1 nM SR solution, followed by drying in air and thermal annealing at 90 °C for 36 h. The diffusion of individual SRB occurring within t
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