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This report presents the groundbreaking finding that the addition of light CaCO3 results in a considerable improvement in pullulan production. Microencapsulation of chlorophyll, following its extraction, was carried out using different carrier agents. Subsequent to the in vitro digestion procedure, the bioaccessibility of chlorophyll in each encapsulation system was carried out. Significant alterations to the zeta potential, particle size, and PDI were observed subsequent to the micellarization