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Stimulated cellular growth, differentiation, and survival are key aspects of the enhanced bone healing facilitated by the synergistic interaction of scaffolds with recombinant human epidermal growth factor (rhEGF) protein. We explored the biopotentials of an rhEGF-loaded absorbable collagen scaffold (ACS) in a mouse model of calvarial defects, using a plant cell suspension culture system to generate the rhEGF, which presented various systemic advantages. Large-scale and successf