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Single-molecule super-resolution microscopy (SRM) combines single-molecule detection with spatial resolutions tenfold improved over conventional confocal microscopy. These two key advantages make it possible to visualize individual DNA ****tion and damage events within the cellular context of fixed cells. This in turn engenders the ability to decipher variations between individual ****tive and damage species within a single nucleus, elucidating different subpopulations of stress and repair events. Here, we describe the protocol fo