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The investigation of these effects utilized exofactor assays, crystal violet staining, and liquid chromatography-mass spectrometry (LC-MS) metabolomics. In contrast to the untreated P. aeruginosa control group, the addition of L. plantarum cell-free supernatant (5%) and Fructooligosaccharides (FOS) (2%) resulted in a substantial reduction of pyoverdine (PVD) and other metabolites involved in the quorum sensing (QS) pathway, including Pseudomonas autoinducer-2 (PAI-2). The metabolomics study revealed
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