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6 per cent carbapenemases within three hours (P=0.001 for MBL and P0.05 for CHDL) and 65.3 per cent within six hours (P=0.001 for MBL and P0.05 for CHDL). MALDI-TOF MS reliably detected carbapenemase activity within a short span of time, thus helping in tailoring patient therapy. MALDI-TOF MS, once optimized, can prove to be a useful tool for timely detection of carbapenemase production by A. baumannii and consequently in directing appropriate antimicrobial therapy. MALDI-TOF MS reliably detected carbapenemase activity within