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C. elegans flow calibration and fluorescent microscopic imaging are detailed to determine their molecular and/or cellular reactions to chemosensory stimuli. The worm's head-first insertion into the chip's head makes this protocol applicable to amphid neurons as well. Further details on this protocol's operation and practical application are provided in Bruggeman et al.'s 2022 publication. Host-derived fatty acids (FAs) are appropriated by Mycobacterium tuberculosis (Mtb) to su