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To evaluate cranial ultrasound markers during a first-trimester routine ultrasound examination for screening for open spina bifida (OS. Midsagittal and axial images of the fetal head obtained from fetuses with spina bifida and unaffected control fetuses at 11 weeks to 13 weeks 6 days were analyzed retrospectively. The observed markers of the posterior brain included the 4- versus 3-line view, the brain stem (BS)-to-brain stem-occipital bone (BSO distance ratio (BS/BSO, the position relationship between the maxillo-occipital (MO) lin
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