https://www.selleckchem.com/pr....oducts/pha-848125.ht
DNA is packaged with histones to form chromatin that impinges on all nuclear processes, including transcription, ****tion and repair, in the eukaryotic nucleus. A complete understanding of these molecular processes requires analysis of chromatin context in vitro. Here, Drosophila four core histones were produced in a native and unmodified form using wheat germ cell-free protein synthesis. In the assembly reaction, four unpurified core histones and three chromatin assembly factors (dNAP-1, dAcf1 and dISWI) were incubated with templ