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Nevertheless, matrices with a stiffness of 50 kPa, leading to a 66 ng/mL increase in TGF-1 expression, resulted in a decrease in osteogenic gene expression within the ESsT-Cs. The complete lack of osteogenesis observed in CTG-Fs was likely attributable to the presence of endogenous or stress-induced TGF-1 at a concentration of 46 ng/mL. Investigating ESsT-Cs' response to TGF-1 and IGF-1 treatment highlighted