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To analyze long-term effects of antenatal betamethasone (≤16mg, =24mg and 24mg) in preterm twins on infant and childhood morbidity. Retrospective cohort study among 198 preterm twins. Three follow up time points, including a total of 84 outcomes, were evaluated first neonatal examination after birth and in the neonatal period up to 10days after birth using data from the clinic charts; examination from the 21st to the 24th month of life and examination from the 60th to the 64th months, using data from copies of the children's examination